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Spectral Response of Red Lettuce with Zinc Uptake: Pot Experiment in Heavy Metal Contaminated Soil
아연섭취에 따른 적상추의 분광학적 반응: 중금속 오염토양에서의 반응실험
Econ. Environ. Geol. 2019 Apr;52(2):129-39
Published online April 30, 2019;
Copyright © 2019 the Korean society of economic and environmental gelology.

Ji Hye Shin1, Jaehyung Yu2*, Jieun Kim1, Sang-Mo Koh3 and Bum Han Lee3
신지혜1 · 유재형2* · 김지은1 · 고상모3 · 이범한3

1Department of Astronomy, Space Science and Geology, Chungnam National University
2Department of Geology and Earth Environmental Sciences, Chungnam National University
3Convergence research center for development of mineral resources (DMR), Korea Institute of Geoscience and Mineral Resources
1충남대학교 우주·지질학과, 2충남대학교 지질환경과학과, 3한국지질자원연구원 DMR융합연구단
Received January 14, 2019; Revised February 22, 2019; Accepted April 4, 2019.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
This study investigates the spectral response of red lettuce (Lactuca sativa var crispa L.) to Zn concentration. The control group and the experimental groups treated with 1 mM(ZnT1), 5 mM(ZnT2), 10 mM(ZnT3), 50 mM(ZnT4), and 100 mM(ZnT5) were prepared for a pot experiment. Then, Zn concentration and spectral reflectance were measured for the different levels of Zn concentration in red lettuce. The Zn concentration of the control group had the range of 134-181 mg/kg, which was within the normal range of Zn concentration in uncontaminated crops. However, Zn concentration in the experimental group gradually increased with an increase in concentration of Zn injection. The spectral reflectance of red lettuce showed high peak in the red band due to anthocyanin, high reflectance in the infrared band due to the scattering effect of the cell structure, and absorption features associated with water. As Zn concentration in red lettuce leaves increased, the reflectance increased in the green and red bands and the reflectance decreased in the infrared band. The correlation analysis between Zn concentration and spectral reflectance showed that the reflectance of 700-1300 nm had a significant negative correlation with Zn concentration. The spectral band is a wavelength region closely related to the cell structure in the leaf, indicating possible cell destruction of leaf structure due to increased Zn concentration. In particular, 700-800 nm reflectance of the infrared band showed the strongest correlation with the Zn concentration. This study could be used to investigate the heavy metal contamination in soil around mining and agriculture area by spectroscopically recognizing heavy metal pollution of plant.
Keywords : zinc concentration, red lettuce, spectral characteristics, pot experiment


June 2019, 52 (3)